Estrogenic and non-estrogenic effects of bisphenol A and its action mechanism in the zebrafish model: An overview of the past two decades of work.
Environment international
Bisphenol A (BPA) is the most simple and predominant component of the Bisphenol family. BPA is widely present in the environment and the human body as a result of its extensive usage in the plastic and epoxy resins of consumer goods like water bottles, food containers, and tableware. Since the 1930s, when BPA's estrogenic activity was first observed, and it was labeled as a "mimic hormone of E2", studies on the endocrine-disrupting effects of BPA then have been widely conducted. As a top vertebrate model for genetic and developmental studies, the zebrafish has caught tremendous attention in the past two decades. By using the zebrafish, the negative effects of BPA either through estrogenic signaling pathways or non-estrogenic signaling pathways were largely found. In this review, we tried to draw a full picture of the current state of knowledge on the estrogenic and non-estrogenic effects of BPA with their mechanisms of action through the zebrafish model of the past two decades, which may help to fully understand the endocrine-disrupting effects of BPA and its action mechanism, and give a direction for the future studies.
10.1016/j.envint.2023.107976
The neurological toxicity of heavy metals: A fish perspective.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
The causes of neurodegenerative diseases are complex with likely contributions from genetic susceptibility and environmental exposures over an organism's lifetime. In this review, we examine the role that aquatic models, especially zebrafish, have played in the elucidation of mechanisms of heavy metal toxicity and nervous system function over the last decade. Focus is applied to cadmium, lead, and mercury as significant contributors to central nervous system morbidity, and the application of numerous transgenic zebrafish expressing fluorescent reporters in specific neuronal populations or brain regions enabling high-resolution neurodevelopmental and neurotoxicology research.
10.1016/j.cbpc.2017.11.008
Immunotoxicity mechanisms of perfluorinated compounds PFOA and PFOS.
Liang Luyun,Pan Yongling,Bin Lihua,Liu Yu,Huang Wenjun,Li Rong,Lai Keng Po
Chemosphere
Perfluorinated and polyfluorinated compounds (PFASs) are a class of synthetic chemical substances that are widely used in human production and life, such as fire-fighting foams, textiles and clothing, surfactants, and surface protective agents. Perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) are the most abundant and common perfluorinated compounds in biota and humans. Currently, PFOA and PFOS have been listed in the Stockholm Convention on Persistent Organic Pollutants, and their production has been halted in many countries. However, because the high-energy carbon-fluorine bond can make it resistant to hydrolysis, photolysis, microbial degradation, and vertebrate metabolism, PFOA and PFOS show environmental persistence and bioaccumulation and hence, are of great concern to humans and wildlife. PFOA and PFOS have toxic effects on the immune system of the body. This article reviewed the effects of PFOA and PFOS on immune organs such as the spleen, bone marrow, and thymus of mice and zebrafish, and the effects on non-specific immune functions such as the skin barrier, intestinal mucosal barrier, and humoral immunity. We also reviewed the influence of specific immune functions based on cellular immunity, and further summarized the possible immune toxicity mechanisms such as AIM2 inflammasome activation, gene dysregulation, and signal pathway disorders caused by PFOA and PFOS. The aim of this review was to provide a reference for further understanding of the immunotoxicity and the responsible mechanism of PFOA and PFOS.
10.1016/j.chemosphere.2021.132892
Effects of titanium dioxide nanoparticles on lead bioconcentration and toxicity on thyroid endocrine system and neuronal development in zebrafish larvae.
Miao Wei,Zhu Biran,Xiao Xiaohong,Li Ying,Dirbaba Niguse Bekele,Zhou Bingsheng,Wu Hongjuan
Aquatic toxicology (Amsterdam, Netherlands)
Nanoparticles (NPs) have attracted considerable attention because of their wide range of applications. Interactions between heavy metals (e.g., Pb) and NPs in aquatic environments may modify the bioavailability and toxicity of heavy metals. Therefore, this study investigated the influence of NPs (e.g., nano-TiO2) on the bioavailability and toxicity of Pb and its effects in the thyroid endocrine and nervous systems of zebrafish (Danio rerio) larvae. Zebrafish embryos (2-h post-fertilization) were exposed to five concentrations of Pb alone (0, 5, 10, 20, and 30μg/L) or in combination with nano-TiO2 (0.1mg/L) until 6 days post-fertilization. Results showed that the bioconcentration of Pb was significantly enhanced when combined with nano-TiO2 than when used alone. Zebrafish exposure to Pb alone at 30μg/L significantly decreased the thyroid hormone levels (T4 and T3), whereas nano-TiO2 treatment alone did not produce detectable changes. The levels of T4 and T3 were further decreased when Pb was combined with nano-TiO2 than when used alone. The transcription of the thyroid hormone-related factor tg gene was remarkably down-regulated by Pb treatment alone but up-regulated when Pb was combined with nano-TiO2. The significant up-regulation of tshβ gene and the down-regulation of TTR gene expression in the hypothalamic-pituitary-thyroid were observed in Pb with or without nano-TiO2 treatment groups. In addition, the transcription of genes involved in central nervous system (CNS) development (α-tubulin, mbp, gfap and shha) were significantly down-regulated by Pb and nano-TiO2 co-exposure as compared with Pb exposure alone. The locomotion activity analyzes confirmed that nano-TiO2 might enhance the toxicity of Pb to CNS development. These results suggest that nano-TiO2 increase bioconcentration of lead, which lead to the disruption of thyroid endocrine and neuronal system in zebrafish larvae.
10.1016/j.aquatox.2015.02.002
Microplastics and associated contaminants in the aquatic environment: A review on their ecotoxicological effects, trophic transfer, and potential impacts to human health.
Huang Wei,Song Biao,Liang Jie,Niu Qiuya,Zeng Guangming,Shen Maocai,Deng Jiaqin,Luo Yuan,Wen Xiaofeng,Zhang Yafei
Journal of hazardous materials
The microplastic pollution and related ecological impacts in the aquatic environment have attracted global attention over the past decade. Microplastics can be ingested by aquatic organisms from different trophic levels either directly or indirectly, and transferred along aquatic food chains, causing different impacts on life activities of aquatic organisms. In addition, microplastics can adsorb various environmental chemical contaminants and release toxic plastic additives, thereby serving as a sink and source of these associated chemical contaminants and potentially changing their toxicity, bioavailability, and fate. However, knowledge regarding the potential risks of microplastics and associated chemical contaminants (e.g., hydrophobic organic contaminants, heavy metals, plastic additives) on diverse organisms, especially top predators, remains to be explored. Herein, this review describes the effects of microplastics on typical aquatic organisms from different trophic levels, and systematically summarizes the combined effects of microplastics and associated contaminants on aquatic biota. Furthermore, we highlight the research progress on trophic transfer of microplastics and associated contaminants along aquatic food chain. Finally, potential human health concerns about microplastics via the food chain and dietary exposure are discussed. This work is expected to provide a meaningful perspective for better understanding the potential impacts of microplastics and associated contaminants on aquatic ecology and human health.
10.1016/j.jhazmat.2020.124187
Effect of heavy metals on fish larvae deformities: A review.
Sfakianakis D G,Renieri E,Kentouri M,Tsatsakis A M
Environmental research
Heavy metals have been associated with many fish deformities in natural populations and in laboratory produced specimens as well. Deformities in general have devastating effects on fish populations since they affect the survival, the growth rates, the welfare and their external image. Although the embryonic stage in respect to heavy metal exposure has been extensively studied, there is not much information available as to what happens in fish larvae and adults. In the present article, we present the available information on the effect of heavy metals on fish larvae deformities. We also address the need for more research towards the effects of metals on the subsequent life stages in order to assess the long-term consequences of heavy metal poisoning on fish organisms and possibly correlate these consequences with the environmental contamination (use as biomarkers).
10.1016/j.envres.2014.12.014
Studies of the effects of microplastics on aquatic organisms: What do we know and where should we focus our efforts in the future?
de Sá Luís Carlos,Oliveira Miguel,Ribeiro Francisca,Rocha Thiago Lopes,Futter Martyn Norman
The Science of the total environment
The effects of microplastics (MP) on aquatic organisms are currently the subject of intense research. Here, we provide a critical perspective on published studies of MP ingestion by aquatic biota. We summarize the available research on MP presence, behaviour and effects on aquatic organisms monitored in the field and on laboratory studies of the ecotoxicological consequences of MP ingestion. We consider MP polymer type, shape, size as well as group of organisms studied and type of effect reported. Specifically, we evaluate whether or not the available laboratory studies of MP are representative of the types of MPs found in the environment and whether or not they have reported on relevant groups or organisms. Analysis of the available data revealed that 1) despite their widespread detection in field-based studies, polypropylene, polyester and polyamide particles were under-represented in laboratory studies; 2) fibres and fragments (800-1600 μm) are the most common form of MPs reported in animals collected from the field; 3) to date, most studies have been conducted on fish; knowledge is needed about the effects of MPs on other groups of organisms, especially invertebrates. Furthermore, there are significant mismatches between the types of MP most commonly found in the environment or reported in field studies and those used in laboratory experiments. Finally, there is an overarching need to understand the mechanism of action and ecotoxicological effects of environmentally relevant concentrations of MPs on aquatic organism health.
10.1016/j.scitotenv.2018.07.207
Zebrafish as a model system to study toxicology.
Dai Yu-Jie,Jia Yong-Fang,Chen Na,Bian Wan-Ping,Li Qin-Kai,Ma Yan-Bo,Chen Yan-Ling,Pei De-Sheng
Environmental toxicology and chemistry
Monitoring and assessing the effects of contaminants in the aquatic eco-environment is critical in protecting human health and the environment. The zebrafish has been widely used as a prominent model organism in different fields because of its small size, low cost, diverse adaptability, short breeding cycle, high fecundity, and transparent embryos. Recent studies have demonstrated that zebrafish sensitivity can aid in monitoring environmental contaminants, especially with the application of transgenic technology in this area. The present review provides a brief overview of recent studies on wild-type and transgenic zebrafish as a model system to monitor toxic heavy metals, endocrine disruptors, and organic pollutants for toxicology. The authors address the new direction of developing high-throughput detection of genetically modified transparent zebrafish to open a new window for monitoring environmental pollutants.
10.1002/etc.2406
Effects of Virgin Micro- and Nanoplastics on Fish: Trends, Meta-Analysis, and Perspectives.
Jacob Hugo,Besson Marc,Swarzenski Peter W,Lecchini David,Metian Marc
Environmental science & technology
Environmental plastic pollution is a major ecological and societal concern today. Over the past decade, a broad range of laboratory and experimental studies have complemented field observations in the hope of achieving a better understanding of the fate and impact of micro- and/or nanoplastics (MP/NP) on diverse organisms (e.g., birds, fish, and mammals). However, plastic pollution remains challenging to monitor in the environment and to control under laboratory conditions, and plastic particles are often naturally or experimentally co-contaminated with diverse chemical pollutants. Therefore, our understanding of the effects of virgin MP/NP in freshwater and marine fish is still limited. Here, we performed a systematic review of the most up-to-date literature on the effects of virgin MP/NP on fish under laboratory conditions. A total of 782 biological endpoints investigated in 46 studies were extracted. Among these endpoints, 32% were significantly affected by exposure to virgin MP/NP. More effects were observed for smaller plastic particles (i.e., size ≤20 μm), affecting fish behavioral and neurological functions, intestinal permeability, metabolism, and intestinal microbiome diversity. In addition, we propose suggestions for new research directions to lead toward innovative, robust, and scientifically sound experiments in this field. This review of experimental studies reveals that the toxicity of virgin MP/NP on fish should be more systematically evaluated using rigorous laboratory-based methods and aims toward a better understanding of the underlying mechanisms of this toxicity to fish.
10.1021/acs.est.9b05995
Gut microbiota of aquatic organisms: A key endpoint for ecotoxicological studies.
Evariste Lauris,Barret Maialen,Mottier Antoine,Mouchet Florence,Gauthier Laury,Pinelli Eric
Environmental pollution (Barking, Essex : 1987)
Gut microbial communities constitute a compartment of crucial importance in regulation of homeostasis of multiple host physiological functions as well as in resistance towards environmental pollutants. Many chemical contaminants were shown to constitute a major threat for gut bacteria. Changes in gut microbiome could lead to alteration of host health. The access to high-throughput sequencing platforms permitted a great expansion of this discipline in human health while data from ecotoxicological studies are scarce and particularly those related to aquatic pollution. The main purpose of this review is to summarize recent body of literature providing data obtained from microbial community surveys using high-throughput 16S rRNA sequencing technology applied to aquatic ecotoxicity. Effects of pesticides, PCBs, PBDEs, heavy metals, nanoparticles, PPCPs, microplastics and endocrine disruptors on gut microbial communities are presented and discussed. We pointed out difficulties and limits provided by actual methodologies. We also proposed ways to improve understanding of links between changes in gut bacterial communities and host fitness loss, along with further applications for this emerging discipline.
10.1016/j.envpol.2019.02.101
Role of germ-free animal models in understanding interactions of gut microbiota to host and environmental health: A special reference to zebrafish.
Jia Pan-Pan,Junaid Muhammad,Wen Ping-Ping,Yang Yi-Fan,Li Wei-Guo,Yang Xian-Guang,Pei De-Sheng
Environmental pollution (Barking, Essex : 1987)
Numerous pieces of evidence documented the importance of gut microbiota in regulating human health and evaluating the toxicity of environmental pollutants, which are closely related to the host health in various aspects, including nutrition, energy translation, metabolism, pathogen resistance, and immune function. A variety of environmental factors can disrupt gut microbiota and their functions, and inevitably cause immune diseases, obesity and diabetes. However, deciphering the inner mechanisms involved in the functional interaction of gut microbes with host health is still needed extensive investigations. This review focused on the essential roles of intestinal microbes in host-related diseases and highlighted the development and applications of germ-free (GF) animal models, mainly zebrafish. Moreover, the generation, immunity characters, advantages and challenges of GF zebrafish models were also summarized. Importantly, the composition and isolation of zebrafish gut bacteria for further application and toxicity evaluation of aquatic environmental pollutants were also discussed. In conclusion, GF zebrafish play irreplaceable roles in understanding the potential functions and responses of customized microbiota towards human and environmental health implications.
10.1016/j.envpol.2021.116925
Zebrafish in Toxicology and Environmental Health.
Current topics in developmental biology
As manufacturing processes and development of new synthetic compounds increase to keep pace with the expanding global demand, environmental health, and the effects of toxicant exposure are emerging as critical public health concerns. Additionally, chemicals that naturally occur in the environment, such as metals, have profound effects on human and animal health. Many of these compounds are in the news: lead, arsenic, and endocrine disruptors such as bisphenol A have all been widely publicized as causing disease or damage to humans and wildlife in recent years. Despite the widespread appreciation that environmental toxins can be harmful, there is limited understanding of how many toxins cause disease. Zebrafish are at the forefront of toxicology research; this system has been widely used as a tool to detect toxins in water samples and to investigate the mechanisms of action of environmental toxins and their related diseases. The benefits of zebrafish for studying vertebrate development are equally useful for studying teratogens. Here, we review how zebrafish are being used both to detect the presence of some toxins as well as to identify how environmental exposures affect human health and disease. We focus on areas where zebrafish have been most effectively used in ecotoxicology and in environmental health, including investigation of exposures to endocrine disruptors, industrial waste byproducts, and arsenic.
10.1016/bs.ctdb.2016.10.007
The uses of zebrafish (Danio rerio) as an in vivo model for toxicological studies: A review based on bibliometrics.
Ecotoxicology and environmental safety
An in vivo model is necessary for toxicology. This review analyzed the uses of zebrafish (Danio rerio) in toxicology based on bibliometrics. Totally 56,816 publications about zebrafish from 2002 to 2023 were found in Web of Science Core Collection, with Toxicology as the top 6 among all disciplines. Accordingly, the bibliometric map reveals that "toxicity" has become a hot keyword. It further reveals that the most common exposure types include acute, chronic, and combined exposure. The toxicological effects include behavioral, intestinal, cardiovascular, hepatic, endocrine toxicity, neurotoxicity, immunotoxicity, genotoxicity, and reproductive and transgenerational toxicity. The mechanisms include oxidative stress, inflammation, autophagy, and dysbiosis of gut microbiota. The toxicants commonly evaluated by using zebrafish model include nanomaterials, arsenic, metals, bisphenol, and dioxin. Overall, zebrafish provide a unique and well-accepted model to investigate the toxicological effects and mechanisms. We also discussed the possible ways to address some of the limitations of zebrafish model, such as the combination of human organoids to avoid species differences.
10.1016/j.ecoenv.2024.116023
A review of the toxicity in fish exposed to antibiotics.
Yang Changwon,Song Gwonhwa,Lim Whasun
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
Antibiotics are widely used in the treatment of human and veterinary diseases and are being used worldwide in the agriculture industry to promote livestock growth. However, a variety of antibiotics that are found in aquatic environments are toxic to aquatic organisms. Antibiotics are not completely removed by wastewater treatment plants and are therefore released into aquatic environments, which raises concern about the destruction of the ecosystem owing to their non-target effects. Since antibiotics are designed to be persistent and work steadily in the body, their chronic toxicity effects have been studied in aquatic microorganisms. However, research on the toxicity of antibiotics in fish at the top of the aquatic food chain is relatively poor. This paper summarizes the current understanding of the reported toxicity studies with antibiotics in fish, including zebrafish, to date. Four antibiotic types; quinolones, sulfonamides, tetracyclines, and macrolides, which are thought to be genetically toxic to fish have been reported to bioaccumulate in fish tissues, as well as in aquatic environments such as rivers and surface water. The adverse effects of these antibiotics are known to cause damage to developmental, cardiovascular, and metabolic systems, as well as in altering anti-oxidant and immune responses, in fish. Therefore, there are serious concerns about the toxicity of antibiotics in fish and further research and strategies are needed to prevent them in different regions of the world.
10.1016/j.cbpc.2020.108840
Review of environmental effects of oxybenzone and other sunscreen active ingredients.
Schneider Samantha L,Lim Henry W
Journal of the American Academy of Dermatology
With increasing awareness regarding the risks of sunburn, photoaging, and skin cancer, the use of sunscreens has increased. Organic and inorganic filters are used in sunscreen products worldwide. Concerns have been raised regarding the environmental effects of commonly used organic ultraviolet (UV) filters, including oxybenzone (benzophenone-3), 4-methylbenzylidene camphor, octocrylene, and octinoxate (ethylhexyl methoxycinnamate). Studies have identified UV filters such as oxybenzone, octocrylene, octinoxate, and ethylhexyl salicylate in almost all water sources around the world and have commented that these filters are not easily removed by common wastewater treatment plant techniques. Additionally, in laboratory settings, oxybenzone has been implicated specifically as a possible contributor to coral reef bleaching. Furthermore, UV filters such as 4-methylbenzylidene camphor, oxybenzone, octocrylene, and octinoxate have been identified in various species of fish worldwide, which has possible consequences for the food chain. As dermatologists, it is important for us to continue to emphasize the public health impact of excessive sun exposure and advise our patients about proper photoprotection practice, which consists of seeking shade, wearing photoprotective clothing (including hats and sunglasses), and applying appropriate sunscreens.
10.1016/j.jaad.2018.06.033
Zebrafish: An emerging model to study microplastic and nanoplastic toxicity.
Bhagat Jacky,Zang Liqing,Nishimura Norihiro,Shimada Yasuhito
The Science of the total environment
Microplastics (MPs) and nanoplastics (NPs) have received global concern due to its widespread contamination, ingestion in aquatic organisms and the ability to cross the biological barrier. However, our understanding of its bioaccumulation, toxicity, and interaction with other environmental pollutants is limited. Zebrafish is increasingly used to study the bioaccumulation and toxicity of environmental contaminants because of their small size, ease of breed, short life cycle and inexpensive maintenance. The transparent nature of zebrafish embryo and larvae provides excellent experimental advantages over other model organisms in studying the localization of fluorescent-labeled MPs/NPs particles. Zebrafish outplays the traditional rodent models with the availability of transgenic lines, high-throughput sequencing and genetic similarities to humans. All these characteristics provide an unprecedented opportunity to investigate the toxicity of MPs/NPs and associated contaminants. This review summarizes the existing literature on MPs/NPs research in zebrafish and suggests a path forward for future research.
10.1016/j.scitotenv.2020.138707
Fish to learn: insights into the effects of environmental chemicals on eye development and visual function in zebrafish.
Environmental science and pollution research international
Vision is the most essential sense system for the human being. Congenital visual impairment affects millions of people globally. It is increasingly realized that visual system development is an impressionable target of environmental chemicals. However, due to inaccessibility and ethical issues, the use of humans and other placental mammals is constrained, which limits our better understanding of environmental factors on ocular development and visual function in the embryonic stage. Therefore, as complementing laboratory rodents, zebrafish has been the most frequently employed to understand the effects of environmental chemicals on eye development and visual function. One of the major reasons for the increasing use of zebrafish is their polychromatic vision. Zebrafish retinas are morphologically and functionally analogous to those of mammalian, as well as evolutionary conservation among vertebrate eye. This review provides an update on harmful effects from exposure to environmental chemicals, involving metallic elements (ions), metal-derived nanoparticles, microplastics, nanoplastics, persistent organic pollutants, pesticides, and pharmaceutical pollutants on the eye development and visual function in zebrafish embryos. The collected data provide a comprehensive understanding of environmental factors on ocular development and visual function. This report highlights that zebrafish is promising as a model to identify hazardous toxicants toward eye development and is hopeful for developing preventative or postnatal therapies for human congenital visual impairment.
10.1007/s11356-023-27629-3
Evaluation of toxicity of Personal Care Products (PCPs) in freshwaters: Zebrafish as a model.
Environmental toxicology and pharmacology
Personal care products (PCPs) are part of the large and growing family of emerging contaminants (ECs). Many daily products such as sunscreens, toothpaste, make-up products, perfume, and others, fall under this definition, and their use is increasing exponentially. Furthermore, the degradation of some components of these products is limited. Indeed, they are able to easily reach and accumulate in aquatic systems, representing a new class of contaminants. Moreover, due to their chemical properties, they can interfere at different biological levels, and for this reason, they need to be thoroughly investigated. We have reviewed the literature on PCPs, with a special focus on the adverse effects on the freshwater zebrafish (Danio rerio). The aim of this work is to provide a careful assessment of the toxicity of these compounds, in order to raise awareness for more conscious and responsible use.
10.1016/j.etap.2022.103923
Frontier of toxicology studies in zebrafish model.
Journal of applied toxicology : JAT
Based on the 87 original publications only from quartiles 1 and 2 of Journal Citation Report (JCR) collected by the major academic databases (Science Direct, Web of Science, PubMed, and Wiley) in 2022, the frontier of toxicology studies in zebrafish model is summarized. Herewith, a total of six aspects is covered such as developmental, neurological, cardiovascular, hepatic, reproductive, and immunizing toxicities. The tested samples involve chemicals, drugs, new environmental pollutants, nanomaterials, and its derivatives, along with those related mechanisms. This report may provide a frontier focus benefit to researchers engaging in a zebrafish model for environment, medicine, food, and other fields.
10.1002/jat.4543
Effects of common environmental endocrine-disrupting chemicals on zebrafish behavior.
Tao Yue,Li Zixu,Yang Yang,Jiao Yaqi,Qu Jianhua,Wang Yifan,Zhang Ying
Water research
Environmental endocrine-disrupting chemicals (EDCs), a type of exogenous organic pollutants, are ubiquitous in natural aquatic environments. Therefor, this review focused on the use of the zebrafish as a model to explore the effect of different EDCs on behavior, as well as the molecular mechanisms that drive these effects. Furthermore, our study summarizes the current knowledge on the neuromodulatory effects of different EDCs in zebrafish. This study also reviews the current state of zebrafish behavior research, in addition to the potential mechanisms of single and mixed pollutant-driven behavioral dysregulation at the molecular level, as well as the applications of zebrafish behavior experiments for neuroscience research. This review broadens our understanding of the influence of EDCs on zebrafish behavior and provides guidance for future research.
10.1016/j.watres.2021.117826
Impact of pharmaceutical products on zebrafish: An effective tool to assess aquatic pollution.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
Overuse of pharmaceuticals products (PPs) and sometimes ineffective wastewater purification systems have led to the accumulation of these residues in aquatic systems. Raising concerns about the likely harmful effects of these substances both to ecosystems and to human health. Animals as model organisms are nowadays increasingly used to track the health of environmental system around the world. They can be used to understand biological processes, to obtain information on the health status of the environment, and to better understand the effects of xenobiotics on organisms. Among model organisms, the zebrafish (Danio rerio) is one of the best models for studying evolution biology, cancer, toxicology, drug discovery, and genetics. This fish is a multipurpose model organism, due to its easy of maintenance and keeping and the transparency of the embryo during the early stages of development. In this paper, the toxicological effects of typical PPs, and their effects on zebrafish are reviewed. Many PPs have been found to be toxic or even fatal to zebrafish. Showing how these pharmaceuticals compound can affect zebrafish from the larval stage and even in the adult stage. Zebrafish is thus a model for how we can better understand how medications affect not only individual fish but the entire aquatic ecosystem, bringing about perturbations in their behaviour and putting their survival at risk.
10.1016/j.cbpc.2022.109439
Neurotoxicity of nanoparticles: Insight from studies in zebrafish.
Ecotoxicology and environmental safety
Nanoparticles are widely used in industry and personal care, and they inevitably end up in people's bodies and the environment. The widespread use of nanoparticles has raised new concerns about their neurotoxicity, as nanoparticles can enter the nervous system by blood-brain barrier. In neurotoxicity testing, the zebrafish provides powerful tools to overcome the limitations of other models. This paper will provide a comprehensive review of the power of zebrafish in neurotoxicity tests and the neurotoxic effects of nanoparticles, including inorganic, organic, and metal-based nanoparticles, on zebrafish from different perspectives. Such information can be used to predict not only the effects of nanoparticles on other species exposed to the aquatic environment but also the neurotoxicity of nanoparticles in humans.
10.1016/j.ecoenv.2022.113896
The zebrafish embryotoxicity test (ZET) for nanotoxicity assessment: from morphological to molecular approach.
Pereira Aryelle Canedo,Gomes Tânia,Ferreira Machado Mônica Rodrigues,Rocha Thiago Lopes
Environmental pollution (Barking, Essex : 1987)
Nanotechnology and use of nanomaterials (NMs) improve life quality, economic growth and environmental health. However, the increasing production and use of NMs in commercial products has led to concerns about their potential toxicity on human and environment health, as well as its toxicological classification and regulation. In this context, there is an urgent need to standardize and validate procedures for nanotoxicity testing. Since the zebrafish embryotoxicity test (ZET) has been indicated as a suitable approach for the toxicity assessment of traditional and emergent pollutants, the aim of this review is to summarize the existing literature on embryotoxic and teratogenic effects of NMs on zebrafish. In addition, morphological changes in zebrafish embryos induced by NMs were classified in four reaction models, allowing classification of the mode of action and toxicity of different types of NM. Revised data showed that the interaction and bioaccumulation of NMs on zebrafish embryos were associated to several toxic effects, while the detoxification process was limited. In general, NMs induced delayed hatching, circulatory changes, pigmentation and tegumentary alterations, musculoskeletal disorders and yolk sac alterations on zebrafish embryos. Recommendations for nanotoxicological tests are given, including guidance for future research. This review reinforces the use of the ZET as a suitable approach to assess the health risks of NM exposure.
10.1016/j.envpol.2019.06.100
Zebrafish as a Vertebrate Model System to Evaluate Effects of Environmental Toxicants on Cardiac Development and Function.
Sarmah Swapnalee,Marrs James A
International journal of molecular sciences
Environmental pollution is a serious problem of the modern world that possesses a major threat to public health. Exposure to environmental pollutants during embryonic development is particularly risky. Although many pollutants have been verified as potential toxicants, there are new chemicals in the environment that need assessment. Heart development is an extremely sensitive process, which can be affected by environmentally toxic molecule exposure during embryonic development. Congenital heart defects are the most common life-threatening global health problems, and the etiology is mostly unknown. The zebrafish has emerged as an invaluable model to examine substance toxicity on vertebrate development, particularly on cardiac development. The zebrafish offers numerous advantages for toxicology research not found in other model systems. Many laboratories have used the zebrafish to study the effects of widespread chemicals in the environment on heart development, including pesticides, nanoparticles, and various organic pollutants. Here, we review the uses of the zebrafish in examining effects of exposure to external molecules during embryonic development in causing cardiac defects, including chemicals ubiquitous in the environment and illicit drugs. Known or potential mechanisms of toxicity and how zebrafish research can be used to provide mechanistic understanding of cardiac defects are discussed.
10.3390/ijms17122123