Ambient temperature affects thrombotic potential at rest and following exercise. Nagelkirk Paul R,Hogan Kyla B,Hoare Joanna M Thrombosis research INTRODUCTION:During exercise, ischemic risk increases, possibly due to changes in coagulation and fibrinolytic activity. Previous research suggests ambient temperature affects resting thrombotic potential, but the effect of heat and cold on hemostasis during exercise is unknown. The purpose of this study was to assess changes in coagulation and fibrinolysis during maximal exercise in hot and cold temperatures, and to compare those responses to exercise under temperate conditions. MATERIALS & METHODS:Fifteen healthy men completed maximal exercise tests in hot (30°C), temperate (20°C) and cold (5° - 8°C) temperatures. Blood samples were obtained before and immediately after exercise and analyzed for concentrations of thrombin-antithrombin III (TAT), active tissue plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1). Results were analyzed by ANOVA. RESULTS:A main effect of time was observed for TAT (temperate=1.71 ± 0.82 - 2.61 ± 0.43 ng/ml, hot=1.81 ± 0.73 - 2.62 ± 0.67 ng/ml, cold=2.33 ± 0.65 - 2.89 ± 0.81 ng/ml, PRE to POST, respectively) and tPA activity (temperate=0.72 ± 0.44 - 2.71 ± 0.55 IU/ml, hot=0.72 ± 0.38 - 2.64 ± 0.61 IU/ml, cold=0.86 ± 0.45 - 2.65 ± 0.77 IU/ml, PRE to POST, respectively). A trend was observed for the PAI-1 response to exercise (temperate=14.5 ± 23.7 - 12.3 ± 20.2I U/ml, hot=15.1 ± 26.5 - 10.0 ± 15.1 IU/ml, cold=10.5 ± 10.4 - 7.9 ± 9.7 IU/ml, PRE to POST, respectively, p=0.08). TAT concentrations were significantly higher in cold compared to temperate and hot conditions. CONCLUSION:Coagulation potential is elevated during exposure to cold temperatures. These data suggest that risk of an ischemic event may be elevated in the cold. 10.1016/j.thromres.2011.10.015

Ambient temperature influences the neural benefits of exercise. Maynard Mark E,Chung Chasity,Comer Ashley,Nelson Katharine,Tran Jamie,Werries Nadja,Barton Emily A,Spinetta Michael,Leasure J Leigh Behavioural brain research Many of the neural benefits of exercise require weeks to manifest. It would be useful to accelerate onset of exercise-driven plastic changes, such as increased hippocampal neurogenesis. Exercise represents a significant challenge to the brain because it produces heat, but brain temperature does not rise during exercise in the cold. This study tested the hypothesis that exercise in cold ambient temperature would stimulate hippocampal neurogenesis more than exercise in room or hot conditions. Adult female rats had exercise access 2h per day for 5 days at either room (20 °C), cold (4.5 °C) or hot (37.5 °C) temperature. To label dividing hippocampal precursor cells, animals received daily injections of BrdU. Brains were immunohistochemically processed for dividing cells (Ki67+), surviving cells (BrdU+) and new neurons (doublecortin, DCX) in the hippocampal dentate gyrus. Animals exercising at room temperature ran significantly farther than animals exercising in cold or hot conditions (room 1490 ± 400 m; cold 440 ± 102 m; hot 291 ± 56 m). We therefore analyzed the number of Ki67+, BrdU+ and DCX+ cells normalized for shortest distance run. Contrary to our hypothesis, exercise in either cold or hot conditions generated significantly more Ki67+, BrdU+ and DCX+ cells compared to exercise at room temperature. Thus, a limited amount of running in either cold or hot ambient conditions generates more new cells than a much greater distance run at room temperature. Taken together, our results suggest a simple means by which to augment exercise effects, yet minimize exercise time. 10.1016/j.bbr.2015.11.017

Effect of Pre-Exercise Caffeine Intake on Endurance Performance and Core Temperature Regulation During Exercise in the Heat: A Systematic Review with Meta-Analysis. Sports medicine (Auckland, N.Z.) BACKGROUND:Heat is associated with physiological strain and endurance performance (EP) impairments. Studies have investigated the impact of caffeine intake upon EP and core temperature (C) in the heat, but results are conflicting. There is a need to systematically determine the impact of pre-exercise caffeine intake in the heat. OBJECTIVE:To use a meta-analytical approach to determine the effect of pre-exercise caffeine intake on EP and C in the heat. DESIGN:Systematic review with meta-analysis. DATA SOURCES:Four databases and cross-referencing. DATA ANALYSIS:Weighted mean effect summaries using robust variance random-effects models for EP and C, as well as robust variance meta-regressions to explore confounders. STUDY SELECTION:Placebo-controlled, randomized studies in adults (≥ 18 years old) with caffeine intake at least 30 min before endurance exercise ≥ 30 min, performed in ambient conditions ≥ 27 °C. RESULTS:Respectively six and 12 studies examined caffeine's impact on EP and C, representing 52 and 205 endurance-trained individuals. On average, 6 mg/kg body mass of caffeine were taken 1 h before exercises of ~ 70 min conducted at 34 °C and 47% relative humidity. Caffeine supplementation non-significantly improved EP by 2.1 ± 0.8% (95% CI - 0.7 to 4.8) and significantly increased the rate of change in C by 0.10 ± 0.03 °C/h (95% CI 0.02 to 0.19), compared with the ingestion of a placebo. CONCLUSION:Caffeine ingestion of 6 mg/kg body mass ~ 1 h before exercise in the heat may provide a worthwhile improvement in EP, is unlikely to be deleterious to EP, and trivially increases the rate of change in C. 10.1007/s40279-022-01692-1